Freeze-all: enhanced outcomes with cryopreservation at the blastocyst stage versus pronuclear stage using slow-freeze techniques
نویسندگان
چکیده
منابع مشابه
Cryopreservation of biopsied embryos at the blastocyst stage.
BACKGROUND The availability of an efficient cryopreservation program is especially important in the case of embryos that have undergone blastomere biopsy for PGD. Unfortunately, the freezing/thawing of biopsied embryos has given disappointing results when performed at the cleavage stage. In this study, embryos diagnosed as normal after PGD were grown to the blastocyst stage, frozen and thawed f...
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This study was designed to investigate the dynamics of the paternal genome demethylation in pronuclear-stage bovine zygotes produced either by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) using freeze-thawed (FT) as well as freeze-dried (FD) bull sperm stored at +4 or -196 C for one year. Zygotes were fixed and immunostained using anti-5-methyl-cytosin at 8, 10, 14 an...
متن کاملEstablishment of Blastocyst Production System with Freeze-dried Bull Spermatozoa
25 Introduction 25 Materials and methods 27 Results 32 Discussion 35 Chapter 4: Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center 39 Abstract 39 Introduction 39 Materials and methods 40 Results 43 Discussion 47 Chapter 5: Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa 49 Abstract 49 Introdu...
متن کاملTechnique to 'Map' Chromosomal Mosaicism at the Blastocyst Stage.
The purpose of this study was to identify a technique that allows for comprehensive chromosome screening (CCS) of individual cells within human blastocysts along with the approximation of their location in the trophectoderm relative to the inner cell mass (ICM). This proof-of-concept study will allow for a greater understanding of chromosomal mosaicism at the blastocyst stage and the mechanisms...
متن کاملLive birth from a frozen-thawed pronuclear stage embryo almost 20 years after its cryopreservation.
OBJECTIVE To report a live birth after transfer of cryopreserved pronuclear embryos in cryostorage for almost 20 years. DESIGN Case report. SETTING Academic IVF center. PATIENT(S) A 42-year-old female patient with low ovarian reserve receiving donated embryos. These embryos were the result of an anonymous donation from an infertile couple who had conceived during her IVF treatment. INTE...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2010
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2010.04.008